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Capitoli
- 00:05Titolo
- 01:21Basic Design of the Convective Thermocycler
- 02:48Preparation and Loading of the PCR Reaction Mixture
- 03:42Running the Reaction in the Convective Thermocycler
- 04:25Performing Gel Electrophoresis Analysis
- 05:31Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
- 08:33Conclusion
We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.
Tags
PCR ThermocyclingRapid PCRMicroscale Thermal ConvectionPolymerase Chain ReactionThermal ConvectionPCR Thermocycling HardwareThermoelectric HeatersReaction SpeedElectrical PowerPortable FormatConvective FlowsBuoyancy Driven InstabilitySpatial Temperature GradientReactor GeometryDenaturing StageAnnealing StageExtension Stage
