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前肿瘤反应性T细胞的体外扩增Bryostatin 1/Ionomycin手段和共同的γ链细胞因子配方
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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前肿瘤反应性T细胞的体外扩增Bryostatin 1/Ionomycin手段和共同的γ链细胞因子配方

DOI:
10.3791/2381-v

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07:20 min

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January 14, 2011

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, , , ,
1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Capitoli

  • 00:05Titolo
  • 00:55Introduction
  • 01:48Mouse Model of Breast Cancer
  • 02:14Isolation of Lymphocytes
  • 03:44Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis
  • 04:58Determine Tumor-Specificity of the ex vivo Expanded T Cells
  • 05:47Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function
  • 06:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

为有效的协议<em>体外</em>描述从肿瘤引流淋巴结或其他淋巴组织的继发性肿瘤轴承主机的肿瘤反应性T细胞的扩张。该协议有选择地扩大肿瘤特异性T细胞过继免疫治疗乳腺癌。

Tags

Ex Vivo ExpansionTumor-reactive T CellsBryostatin 1/IonomycinCommon Gamma Chain Cytokines FormulationBreast CancerImmune ResponsesAdoptive ImmunotherapyTumor-specific T CellsTumor AntigensIL-2IL-7IL-15IL-21FVBN202 Transgenic Mouse ModelHER-2/neu Positive Breast CarcinomaAdoptive T Cell Therapy

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