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在哺乳动物细胞通过显微注射mRNA的核出口动力学分析
JoVE Journal
Biologia
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JoVE Journal Biologia
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
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在哺乳动物细胞通过显微注射mRNA的核出口动力学分析

DOI:
10.3791/2387-v

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11:32 min

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December 04, 2010

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, , , ,
1Department of Biochemistry,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:23Preparation for Microinjection
  • 02:41Intranuclear Microinjection
  • 05:46Cell Fixation and Staining
  • 08:48Representative Results for mRNA Nuclear Export Kinetics
  • 10:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一个实验，采用显微注射的力量，再加上用荧光<em>原位</em>杂交为了准确地测量哺乳动物体细胞中的mRNA的核出口动力学。

Tags

MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization

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