È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 00:17Introduction
- 01:17Tools and Reagents
- 02:33Anesthesia Procedure
- 03:13Preparation of Microinjection Pipets
- 07:18Prepping Rat for Surgery
- 09:59Removing Uterus and Exposing Embryos
- 12:11In utero Injection and Electroporation
- 16:49Placing Embryos Back Inside Dam
- 23:46Conclusion
- 26:01End Credits
Tags
In-uteroIntraventricular InjectionElectroporationE15 Mouse EmbryosNeocortex ManipulationProgenitorsLateral VentricleCorticogenesisGene Over-expressionGene KnockdownCellular ProliferationMigrationDifferentiationKnockout StrategiesTemporal WindowVideo ProtocolSurgery PreparationUterine Horns ExposureDNA InjectionProgenitor ElectroporationAnimal Care Post-surgery
