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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
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JoVE Journal Neuroscienze
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

DOI:
10.3791/2393-v

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13:21 min

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November 20, 2010

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, , ,
1 Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,University of Florida

Capitoli

  • 00:00Titolo
  • 00:41Introduction
  • 01:04Animal Dissection
  • 02:25Brain Dissection
  • 04:31Tissue Preparation and Plating Cells
  • 09:04Passaging Neurospheres
  • 11:56Representative Results
  • 12:55Conclusion

Summary

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This video protocol demonstrates the neurosphere assay method to generate and expand neural stem cells from the adult mouse periventricular region, and provides technical insights to ensure one can achieve reproducible neurosphere cultures.

Tags

IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures

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