È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 00:41Introduction
- 01:04Animal Dissection
- 02:25Brain Dissection
- 04:31Tissue Preparation and Plating Cells
- 09:04Passaging Neurospheres
- 11:56Representative Results
- 12:55Conclusion
Tags
IsolationExpansionAdult MouseNeural Stem CellsNeurosphere AssayPutativeMurine BrainReynolds And WeissChemically Defined Serum-free Culture SystemGrowth Factor Responsive Precursor CellsEpidermal Growth FactorBasic Fibroblastic Growth FactorColoniesUndifferentiated CellsNeurospheresSubculturedPool Of Neural Stem CellsDifferentiateThree Major Cell TypesCNSNeuronsAstrocytesOligodendrocytesInvaluable ToolIn Vitro StudiesTherapeutic PurposesVideo DemonstrationPeriventricular RegionReproducible Neurosphere Cultures
