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Journal / Biology / Detection of Functional Matrix Metalloproteinases …
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Biology

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Detection of Functional Matrix Metalloproteinases by Zymography
 

Detection of Functional Matrix Metalloproteinases by Zymography

Article DOI: 10.3791/2445-v • 09:31 min • November 8th, 2010
November 8th, 2010 •
1, 1
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

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This protocol describes an activity-based assay for detecting matrix metalloproteinases in culture supernatants or body fluids.

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Functional Matrix Metalloproteinases Zymography MMPs Zinc-containing Endopeptidases Peptide Bonds Collagenases Gelatinases MT-MMP Stromelysins Matrilysins Cell Invasion Cartilage Degradation Tissue Remodeling Wound Healing Embryogenesis Rheumatoid Arthritis Cancer Chronic Obstructive Pulmonary Disease MMP-2 Gelatinase A Type IV Collagenase Cell Culture Supernatants Semi-quantitative Technique Polyacrylamide Gel Sodium Dodecyl Sulfate (SDS) Gelatin Substrate
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