Rivista
/
Neuroscienze
/
Organotypic Hippocampal Slice Cultures
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Organotypic Hippocampal Slice Cultures

Organotypic Hippocampal Slice Cultures

DOI:
10.3791/2462-v

•

08:20 min

•

February 03, 2011

•

,
1Department of Physiology and Biophysics,University of Washington School of Medicine

Capitoli

  • 00:05Titolo
  • 00:37Introduction
  • 01:02Preparation of Equipment and Dissection Area
  • 03:12Dissection of the Hippocampus from a Postnatal Rat Pup
  • 06:08Culturing Hippocampal Slices
  • 07:10Examples of Hippocampal Slice Cultures
  • 07:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.

Tags

Organotypic Hippocampal Slice CulturesLimbic SystemLong-term MemorySpatial NavigationHippocampal NeuronsLong-term Potentiation (LTP)Learning And MemorySynaptic TransmissionSynaptic PlasticityGene ManipulationPharmacological InterventionSynaptic ProteinsHippocampal SlicesBrain RegionsViral InfectionBiolisticsBiochemical AssaysImagingElectrophysiological Experiments

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2462?article_type=v

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
check_url/it/2462?article_type=v

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
check_url/it/2462?article_type=v

Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro</em
check_url/it/2462?article_type=v

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
check_url/it/2462?article_type=v

Post-embedding immunogold mærkning af synaptiske proteiner i hippokampalskivekulturer
check_url/it/2462?article_type=v

Organotypiske skivekulturer at studere oligodendrocyt Dynamics og myelinering
check_url/it/2462?article_type=v

Forbundne Helcelle optagelser i Organotypiske hippocampusskiver
check_url/it/2462?article_type=v

Analysen af ​​neurovaskulære Remodeling i Entorhino-hippocampus organotypisk skivekulturer
check_url/it/2462?article_type=v

Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller
check_url/it/2462?article_type=v

Ex Utero Elektroporation og Organotypic skive kulturer af embryonale mus hjerner for Live-Imaging af migrerer GABAergic Interneurons

Read Article