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对于高分辨率延时成像神经细胞迁移的一个器官型产后脑切片检测
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
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对于高分辨率延时成像神经细胞迁移的一个器官型产后脑切片检测

DOI:
10.3791/2486-v

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10:41 min

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December 11, 2010

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, ,
1Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine,North Carolina State University

Capitoli

  • 00:02Titolo
  • 00:06Introduction
  • 00:51Preparation of Culture Dishes
  • 01:57Organotypic Slice Preparation
  • 04:19Explant Preparation, Transplantation and Imaging
  • 08:04Representative Results and Analysis
  • 09:16Conclusion

Summary

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Traduzione automatica

本协议描述了一个器官的切片检测，产后脑和延髓迁徙流时间推移高分辨率成像成神经细胞迁移的优化。

Tags

Organotypic Slice AssayHigh-resolution Time-lapse ImagingNeuronal MigrationPostnatal BrainNeurogenesisProgenitor CellsSubependymal Zone (SEZ)Rostral Migratory Stream (RMS)Olfactory Bulbs (OB)Cellular MechanismsMolecular MechanismsPhysiological MechanismsExtracellular ConditionsMigrating NeuroblastsUniform MovementOriented MovementSpeedy MovementCell Autonomous RegulationNon-autonomous RegulationCross-transplantation Approaches

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