Rivista
/
Biologia
/
异核技术的细胞类型特异性定位分析
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Heterokaryon Technique for Analysis of Cell Type-specific Localization
Please note that all translations are automatically generated. Click here for the English version.

异核技术的细胞类型特异性定位分析

DOI:
10.3791/2488-v

•

09:31 min

•

March 11, 2011

•

, ,
1Department of Chemistry and Biochemistry,Worcester Polytechnic Institute- WPI

Capitoli

  • 00:05Titolo
  • 01:00Introduction
  • 01:34Cell Transfection – Method 1: Nucleofection
  • 03:34Cell Transfection – Method 2: Effectene
  • 05:43Cell Fusion
  • 06:49Fluorescence Microscopy
  • 07:44Analysis of Cell-type Specific Protein Localization
  • 08:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

一个细胞类型特异性的蛋白定位和穿梭核质特性的灵活和有效的方法是描述。这异核体的方法是使用荧光标记的融合蛋白细胞融合后图像蛋白质本地化。该协议是经得起稳态本地化或活细胞成像的基础上更具活力的测定。

Tags

Heterokaryon TechniqueCell Type-specific LocalizationSubcellular TraffickingNucleocytoplasmic ShuttlingProteinsCellular FunctionsNuclear Import/exportTranscriptional RegulationApoptosisCell DivisionDifferentiationTransformationProtein ActivitiesCellular OncogenesisNormal Primary CellsTransformed CellsAltered LocalizationPathwaysDrug TargetingProtein TraffickingShuttling ActivityMammalian CellsHeterokaryon FusionsFluorescence Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2488?article_type=v

一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
check_url/it/2488?article_type=v

可视化的RNA中的本地化爪蟾卵母细胞
check_url/it/2488?article_type=v

调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em
check_url/it/2488?article_type=v

定量健身分析工作流程
check_url/it/2488?article_type=v

Cell的传播分析单细胞显微注射
check_url/it/2488?article_type=v

新技术脱发的定量分析小鼠使用灰度分析
check_url/it/2488?article_type=v

微操作系统技术允许分析骨架调节器的形态发生动力学和营业额
check_url/it/2488?article_type=v

tchip-seq: 细胞专用表观体分析
check_url/it/2488?article_type=v

斑马鱼镜头核定位与功能完整性的评价
check_url/it/2488?article_type=v

小鼠肝脏中细胞类型特异性基因表达分析

Read Article