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Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology
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Bioingegneria
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JoVE Journal Bioingegneria
Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

DOI:
10.3791/2493-v

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07:13 min

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February 03, 2011

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, , ,
1Biomarker Division,BioScale, Inc., 2Bioprocessing Division,BioScale, Inc.

Capitoli

  • 00:00Titolo
  • 01:02Attaching Biotinylated Antibody to the Streptavidin Magnetic Beads
  • 02:42CHO-HCP Assay Development
  • 05:04Acoustic Membrane MicroParticle (AMMP) Assay Representative Results

Summary

Traduzione automatica

Traduzione automatica

Development of custom assays on the ViBE platform for more sensitive, reproducible, automated results in complex matrices is described. The universal cartridge allows assays to be easily adapted for use with custom assays. This versatility enables rapid development and validation of novel assays or automated versions of existing manual assays, exemplified in this video.

Tags

Custom Chinese Hamster Ovary-host Cell Protein AssaysAcoustic Membrane Microparticle TechnologyBioassaysViBE PlatformSandwich ImmunoassaysELISAWestern BlotsSensitivityReproducibilityAutomated OperationProtocolAntigen ConcentrationOscillation FrequencySensor SurfaceFunctionalized Eight Sensor ChipAntibody Labeled Magnetic MicroparticlesHapten Tagged AntibodyAntigen Of InterestBioScale’s Biosensor

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