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使用C2C12成肌细胞的生物能简介实验
JoVE Journal
Biologia
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JoVE Journal Biologia
Bioenergetic Profile Experiment using C2C12 Myoblast Cells
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使用C2C12成肌细胞的生物能简介实验

DOI:
10.3791/2511-v

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07:20 min

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December 06, 2010

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, , , , ,
1Buck Institute for Age Research,Novato, CA, 2Department of Pathology, Center for Free Radical Biology,University of Alabama at Birmingham – UAB, 3Seahorse Bioscience,North Billerica, MA

Capitoli

  • 00:05Titolo
  • 01:04Introduction
  • 02:01Seeding Protocol
  • 03:27Loading Sensor Cartridge and Protocol Commands
  • 04:50Representative Bioenergetic Profiling Results
  • 06:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

在细胞线粒体功能分析的方法的描述。线粒体产生的个人资料提供线粒体的功能，可以在一次实验中测量的四个参数：基础呼吸速率，ATP挂钩呼吸，质子漏，和储备能力。

Tags

Bioenergetic ProfileExperimentC2C12 Myoblast CellsCellular MetabolismMitochondrial DysfunctionObesityDiabetesAgingCancerCardiovascular FunctionSafety ToxicitySubstrate UptakeOxygenGlucoseFatty AcidsGlutamineEnergy ConversionEnzymatically Controlled Oxidation And Reduction ReactionsATP ProductionHeat ReleaseLactate ProductionCO2 ReleaseExtracellular EnvironmentPhysiological State Of CellsOxygen Consumption Rate (OCR)GlycolysisLactate ProductionProtons ReleaseAcidification Rate (ECAR)Seahorse Cell Culture Plates

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