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Use of a Hanging Weight System for Coronary Artery Occlusion in Mice
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JoVE Journal Medicina
Use of a Hanging Weight System for Coronary Artery Occlusion in Mice

Use of a Hanging Weight System for Coronary Artery Occlusion in Mice

DOI:
10.3791/2526-v

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08:30 min

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April 19, 2011

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, ,
1Department of Anesthesiology,University of Colorado Denver

Capitoli

  • 00:05Titolo
  • 01:24Surgical Preparation
  • 04:33Coronary Artery Occlusion
  • 08:06Conclusion

Summary

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A murine model for myocardial ischemia and ischemic preconditioning is an important tool study cardioprotective mechanisms in vivo. Here, we report an easy applicable in situ model for cardiac IP using a hanging-weight system for coronary artery occlusion.

Tags

Hanging Weight SystemCoronary Artery OcclusionMiceMurine StudiesAcute InjuryKnockout MiceIschemic PreconditioningMolecular BasisKnockout Mouse StudiesCardiac Ischemia And ReperfusionTechnical ChallengesVisual Identification Of Coronary ArteryPlacement Of SutureTying Off VesselSupported KnotIntermittent ReperfusionSurgical TraumaImperfect OcclusionBeating HeartChronic Cardiomyopathy

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