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Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
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JoVE Journal Biologia
Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

DOI:
10.3791/253-v

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07:46 min

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August 01, 2007

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,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capitoli

  • 00:04Introduction
  • 00:38Preparing for Heat Transformation
  • 01:14Introducing plasmid DNA into bacteria by heat shock
  • 03:05Plating transformed bacteria on LB agar plate
  • 05:59Assessing the transformation efficiency
  • 06:42Conclusion

Tags

TransformationPlasmid DNAE. ColiHeat Shock MethodBiologia MolecolareForeign PlasmidLigation ProductChemically Competent BacteriaGenlantisIncubationIceDNAHeat ShockSOC MediaTransformed CellsAgitationColoniesTransformation EfficiencyCompetent BacteriaTurbocells3-minute Transformation Protocol

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