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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
JoVE Journal
Biologia
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JoVE Journal Biologia
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DOI:
10.3791/2546-v

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09:27 min

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March 15, 2011

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,
1Department of Pharmacology,University of California, Davis

Capitoli

  • 00:05Titolo
  • 00:51Quality Control Analysis of RNA Sample with 2100 Bioanalyzer
  • 03:05Amplification and Labeling
  • 03:44Microarray Hybridization
  • 05:52Microarray Washing
  • 07:30DNA Microarray Representative Results
  • 09:08Conclusion

Summary

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We demonstrate the use of DNA microarrays for expression profiling of the nervous system. We describe RNA quality control, sample labeling, and array hybridization and scanning.

Tags

DNA MicroarraysSample Quality ControlArray HybridizationScanningGene ActivitiesNervous SystemDevelopment StagesPhysiological StatesPathological StatesTherapy ResponseExperimental TestingHigh Quality RNAAmplificationHybridization ProtocolsAgilent’s 2100 BioanalyzerLab-on-a-chip TechnologyReverse TranscriptionCDNAIn Vitro TranscriptionLabeled CRNADual-color Labeling KitCy3Cy5Agilent’s 4×44 K Arrays

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