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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
 
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Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Article DOI: 10.3791/2561-v • 08:27 min • March 31st, 2011
March 31st, 2011 •
1, 1, 1
1Department of Cancer Biology, University of Massachusetts Medical School

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A rapid, robust way of isolating viable adult epithelial stem cells from human skin is described. The method utilizes enzymatic digestion of skin collagen matrix , followed by plucking of hair follicles and isolation of single cell suspensions or tissue fragments for cell culture.

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Isolation Culture Adult Epithelial Stem Cells Human Skin Homeostasis Self-renewing Tissues Asymmetric Divisions Daughter Cells Stem Cell Phenotype Transit-amplifying Cells Stem Cell Niche Rapid Proliferation Terminal Differentiation Epidermis Hair Follicle Intestine In Vitro Proliferative Potential Label-retaining Cells Tissue-specific Stem Cells Regeneration Of Tissues Multi-potent Stem Cells Rodent Hair Follicle Stem Cells Epidermis Sebaceous Glands Hair Follicles Human Hair Follicle Bulge Region Multi-potentiality Biomedical Research Developmental Biology Differentiation Tumorigenesis Therapeutic Utility
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