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蟹电压敏感染料Stomatogastric神经节神经元的光学成像

JoVE Journal
Neuroscienze

This content is Free Access.

JoVE Journal Neuroscienze

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

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蟹电压敏感染料Stomatogastric神经节神经元的光学成像

DOI:

•

09:36 min

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March 23, 2011

•

Wolfgang Stein, Carola Städele, Peter Andras

¹Institute of Neurobiology,Ulm University, ²School of Computing Science & Institute of Neuroscience,Newcastle University

Capitoli

00:05Titolo
02:11Preparing the Voltage-Sensitive Dye Solution
03:18Preparing the Stomatogastric Ganglion and the Extracellular Recording
03:58Dye Loading by Intracellular Injection
06:35Imaging of Dyed Neurons
08:03Results: Optical and Electrical Recordings from STG Neurons
08:57Conclusion

Summary

Traduzione automatica

在这里，我们目前快速和高分辨率荧光详细的神经元活动的螃蟹stomatogastric节电压敏感染料成像的方法。

Tags

Optical Imaging Neurons Crab Stomatogastric Ganglion Voltage-sensitive Dyes Methodology Neuronal Networks Simultaneous Activity Integral Functionality Network Organization Pattern Generating Neurons Fluorescent Voltage-sensitive Dye Di-8-ANEPPQ MiCAM02 High Speed And High Resolution CCD Camera Imaging System BVAna Imaging Software Electrophysiology Techniques Central Pattern Generator Neural Networks

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