Rivista
/
Neuroscienze
/
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

DOI:
10.3791/2588-v

•

08:57 min

•

July 06, 2011

•

, ,
1Centre of interdisciplinary electron microscopy,École Polytechnique Fédérale de Lausanne

Capitoli

  • 00:05Titolo
  • 01:29Sample Fixation and Resin Embedding
  • 04:00Preparing the Sample for the FIB/SEM
  • 05:55Imaging the FIB/SEM
  • 08:03Results: FIB/SEM Images of Brain Tissue
  • 08:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.

Tags

Focussed Ion Beam MillingScanning Electron MicroscopyFIB/SEM ProtocolAldehydes FixationHeavy Metal StainingOsmium TetroxideUranyl AcetateDehydrationResin InfiltrationUltramicrotomeGlass KnivesBlock PreparationVertical Face MillingBackscattered Electron ImagingSerial ImagingIsotropic VoxelsCell Ultrastructure Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2588?article_type=v

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
check_url/it/2588?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/it/2588?article_type=v

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
check_url/it/2588?article_type=v

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
check_url/it/2588?article_type=v

Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry
check_url/it/2588?article_type=v

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
check_url/it/2588?article_type=v

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
check_url/it/2588?article_type=v

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
check_url/it/2588?article_type=v

Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound
check_url/it/2588?article_type=v

Horizontal Hippocampal Slices of the Mouse Brain

Read Article