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خلفية رفض الإسفار في الرنين وMicrospectroscopy رامان العفوي
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
DOI:

15:04 min

May 18, 2011

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Capitoli

  • 00:05Titolo
  • 06:26Preparation of the Pump Beam (Kerr Beam)
  • 07:46Setting Up the Kerr Gate and Collection System to Maximize the Collected Time-gated Signal
  • 11:14Collecting a Time-gated Spectrum
  • 12:31Representative Time-gated Raman Spectra
  • 14:01Conclusion

Summary

Traduzione automatica

We discuss the construction and operation of a complex nonlinear optical system that uses ultrafast all-optical switching to isolate Raman from fluorescence signals. Using this system we are able to successfully separate Raman and fluorescence signals utilizing pulse energies and average powers that remain biologically safe.

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