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波谱与自发拉曼显微荧光背景的抑制
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Bioingegneria
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JoVE Journal Bioingegneria
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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波谱与自发拉曼显微荧光背景的抑制

DOI:
10.3791/2592-v

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15:04 min

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May 18, 2011

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, , ,
1Center for Biophotonics Science and Technology,University of California, Davis, 2Department of Pathology and Laboratory Medicine,University of California, Davis

Capitoli

  • 00:05Titolo
  • 06:26Preparation of the Pump Beam (Kerr Beam)
  • 07:46Setting Up the Kerr Gate and Collection System to Maximize the Collected Time-gated Signal
  • 11:14Collecting a Time-gated Spectrum
  • 12:31Representative Time-gated Raman Spectra
  • 14:01Conclusion

Summary

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我们讨论一个复杂的非线性光学系统，采用超快全光开关，隔离荧光信号的拉曼的建设和运营。使用这个系统，我们能够成功地分离的拉曼光谱和荧光信号，利用脉冲能量和平均的权力，保持生物安全。

Tags

Fluorescence BackgroundRaman SpectroscopyBiological SamplesUltrafast PulsesPicosecond TimescaleRaman Scattered LightFluorescence SignalsNonlinear Optical SystemOptical Kerr GateLaser PowerRepetition RateNonlinear MediumAll-optical ShutterPeak EfficiencyPulse EnergiesAverage PowersBiologically SafeOptical Power

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