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Bioengineering

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JoVE Journal Bioengineering
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
 
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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Article DOI: 10.3791/2592-v • 15:04 min • May 18th, 2011
May 18th, 2011 •
*1, *1, 1, 1,2
1Center for Biophotonics Science and Technology, University of California, Davis, 2Department of Pathology and Laboratory Medicine, University of California, Davis
* These authors contributed equally

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We discuss the construction and operation of a complex nonlinear optical system that uses ultrafast all-optical switching to isolate Raman from fluorescence signals. Using this system we are able to successfully separate Raman and fluorescence signals utilizing pulse energies and average powers that remain biologically safe.

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Fluorescence Background Raman Spectroscopy Biological Samples Ultrafast Pulses Picosecond Timescale Raman Scattered Light Fluorescence Signals Nonlinear Optical System Optical Kerr Gate Laser Power Repetition Rate Nonlinear Medium All-optical Shutter Peak Efficiency Pulse Energies Average Powers Biologically Safe Optical Power
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