Rivista
/
Biologia
/
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

DOI:
10.3791/2593-v

•

07:13 min

•

April 26, 2011

•

, , , ,
1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University , 2Department of Molecular and Cell Biology,University of Connecticut, 3Department of Pharmaceutical Sciences,University of Connecticut

Capitoli

  • 00:05Titolo
  • 01:26Isolation of Nuclei
  • 03:07Isolation of Nucleosomes by in situ Micrococcal Nuclease Digestion
  • 04:31Nucleosome-ELISA (NU-ELISA)
  • 06:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.

Tags

Post-translational ModificationsNative Intact NucleosomesELISAChromatinNucleosomeHistonesN-terminal TailsAcetylationMethylationPTMsEpigenetic Gene RegulationNucleosome ELISA (NU-ELISA)Global PTM SignaturesEpiproteomic State

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2593?article_type=v

Detection of Functional Matrix Metalloproteinases by Zymography
check_url/it/2593?article_type=v

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
check_url/it/2593?article_type=v

Detection of Histone Modifications in Plant Leaves
check_url/it/2593?article_type=v

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
check_url/it/2593?article_type=v

Identification of Post-translational Modifications of Plant Protein Complexes
check_url/it/2593?article_type=v

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP
check_url/it/2593?article_type=v

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
check_url/it/2593?article_type=v

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
check_url/it/2593?article_type=v

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
check_url/it/2593?article_type=v

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts

Read Article