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Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

DOI:
10.3791/2598-v

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17:59 min

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April 03, 2011

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, , , , ,
1Department of Biomedical Sciences,Cornell University

Capitoli

  • 00:01Titolo
  • 00:05Introduction
  • 00:34Dissection of Tumor
  • 04:11Culturing Lymphoma Cells
  • 10:33Feeding the Cells
  • 11:36Passaging the Cells
  • 11:44Initial Passage
  • 15:17Maintaining Established Cell Lines
  • 16:42Freezing and Recovering the Cell Lines
  • 17:33Cell Recovery from Liquid Nitrogen

Summary

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In this video we demonstrate a protocol to establish mouse thymic lymphoma cell lines. By following this protocol, we have successfully established several T-cell lines from Atm-/- and p53-/- mice with thymic lymphoma.

Tags

Thymic LymphomaT-cell LinesAtm-/- MiceP53-/- MiceGenetically Modified MiceLaboratory AnimalsResearch ToolMurine T-cell LinesInterleukinsProtocolTumorsVisible TumorsHunched PostureLabored BreathingPoor GroomingWastingSusceptible StrainInbred StrainsCD3 ExpressionCD4 ExpressionCD8 ExpressionPassaged

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