Rivista
/
Neuroscienze
/
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

DOI:
10.3791/2600-v

•

10:29 min

•

June 03, 2011

•

, , ,
1Department of Anatomy and Neurobiology,University of Tennessee Health Science Center

Capitoli

  • 00:05Titolo
  • 01:02Introduction
  • 01:30Preparations for Slicing
  • 03:14Slicing the Brain and Preparation for Culture
  • 06:01Transfecting the Slices Using a Gene Gun
  • 07:31Recording from Pyramidal Cells in Slices
  • 09:10Results: Recordings from Neocortical Pyramidal Cells in Organotypic Slice Culture
  • 10:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

This is a protocol to prepare and maintain a neocortical slice preparation in organotypic culture for the purpose of making electrical recordings from pyramidal neurons.

Tags

Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2600?article_type=v

Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
check_url/it/2600?article_type=v

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
check_url/it/2600?article_type=v

Organotypic Hippocampal Slice Cultures
check_url/it/2600?article_type=v

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
check_url/it/2600?article_type=v

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
check_url/it/2600?article_type=v

Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
check_url/it/2600?article_type=v

Organotypiske skivekulturer at studere oligodendrocyt Dynamics og myelinering
check_url/it/2600?article_type=v

Forbundne Helcelle optagelser i Organotypiske hippocampusskiver
check_url/it/2600?article_type=v

Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller
check_url/it/2600?article_type=v

Forberedelse af akut rygmarven skiver til hele-celle Patch-klemme optagelse i Substantia Gelatinosa neuroner

Read Article