Rivista
/
Biologia
/
Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

DOI:
10.3791/2625-v

•

08:32 min

•

March 24, 2011

•

,
1Human Genetics,University of Michigan

Capitoli

  • 00:05Titolo
  • 01:10Mounting Worm Embryos on Agar “Worm Pads”
  • 02:53Setting Up Nomarski DIC Optics for 4D Data Set
  • 05:56Making a GFP Movie
  • 07:17Time-lapse Movies of Early Cell Divisions
  • 07:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

The C. elegans embryo is a powerful system for studying cell biology and development. We present a protocol for live imaging of C. elegans embryos utilizing DIC optics or fluorescence using readily available epifluorescent microscopes and open-source software.

Tags

ImagingC. Elegans EmbryosEpifluorescent MicroscopeOpen Source SoftwareTime-lapse ImagingLiving CellsFluorescent-labeled Reporter ProteinsDifferential Interference Contrast MicroscopyDevelopmental RegulationsCell BiologyDevelopmentCaenorhabiditis Elegans EmbryoCell LineageGenetic ManipulationForward GeneticsReverse GeneticsRNAi-mediated InterferenceTargeted MutagenesisTransgenic AnimalsFluorescently Tagged ProteinsCompound Epifluorescent Microscope

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2625?article_type=v

Generation of Stable Transgenic C. elegans Using Microinjection
check_url/it/2625?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/it/2625?article_type=v

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
check_url/it/2625?article_type=v

Bruk av Time Lapse Mikroskopi å visualisere anoxia-indusert suspendert animasjon i<em> C. elegans</em> Embryoer
check_url/it/2625?article_type=v

Antistoffarging i<em> C. Elegans</em> Ved hjelp av "Freeze-Cracking"
check_url/it/2625?article_type=v

Måle effekten av bakterier på<em> C. Elegans</em> Behavior Ved hjelp av en Egg Retention analysen
check_url/it/2625?article_type=v

En Anoksi-sult Modell for iskemi / Reperfusjon i<em> C. elegans</em
check_url/it/2625?article_type=v

Sette opp et enkelt Lett Sheet mikroskop for<em> I Toto</em> Imaging av<em> C. elegans</em> Utvikling
check_url/it/2625?article_type=v

Arbeidsflyt for High-innhold, Individuell Cell Kvantifisering av Fluorescent Markers fra Universal Microscope data, som støttes av Open Source Software
check_url/it/2625?article_type=v

Sporing og Kvantifisering utviklingsprosesser i<em> C. elegans</em> Ved hjelp av Open-source verktøy

Read Article