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Capitoli
- 00:05Titolo
- 01:32Protein Expression Cysteine Mutation and mRNA Synthesis
- 02:07Oocyte Removal
- 03:36Oocyte Defoculation and mRNA Injection
- 05:05Site-specific Fluorescence Labeling
- 06:07Electrophysiology
- 07:26Anisotropy Measurements and Determination of Distance Constraints
- 09:17Relative Movement of Protein Subunits
- 10:07Results: Current and Fluorescence Changes with Site Specific Fluorophore Labelings
- 11:38Conclusion
Tags
Conformational DynamicsMembrane ProteinsSite-directed Fluorescence LabelingTwo Electrode Voltage Clamp Electrophysiology (TEVC)Ion TransportIon ChannelsIon PumpsTransportersSite-specific Fluorophore LabelingConformational DynamicsVoltage-clamp FluorometryCysteine ReplacementSite-directed Fluorophore LabelingMolecular MotionDistance ConstraintsXenopus Leavis OocytesFunctional ExpressionFluorescence Signal
