È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:06Introduction
- 01:51BiFC Calibration
- 03:04Plating and Transfection of Cells
- 04:37Imaging Cells
- 07:08Monitoring SH3 Interactions of ITSN
- 08:13Conclusion
Tags
Bimolecular Fluorescence ComplementationSubcellular DistributionSignaling ComplexesImmunoprecipitationSpatial LocalizationBiFCProtein ComplexesFluororescent ProteinAmino-terminalCarboxy-terminalProtein FusionInteractionReconstitutionFluorophoreLimitationTransient InteractionsWeak InteractionsKinetic AnalysisComplex DynamicsFlourophore MaturationReal Time InteractionsProtein Fragment Complementation AssayReporter ProteinsGreen Fluorescent Protein VariantsDihydrofolate ReductaseB-lactamaseLuciferaseProtein:protein InteractionsFluorescence Co-localizationFörster Resonance Energy Transfer (FRET)
