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为极化上皮细胞注射质粒的小GTP酶的功能分析
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Biologia
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JoVE Journal Biologia
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
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为极化上皮细胞注射质粒的小GTP酶的功能分析

DOI:
10.3791/2645-v

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09:38 min

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May 31, 2011

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, , ,
1Department of Cell and Molecular Biology,Northwestern University

Capitoli

  • 00:05Titolo
  • 01:27Preparation of DNA and MDCK Cells
  • 02:04Microinjection Procedure and Post-injection Incubations
  • 06:07Surface Staining for Immunofluorescence Analysis
  • 08:21VSVGts045-GFP Control in MDCK Cells
  • 09:05Conclusion

Summary

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本文详细介绍了在极化上皮细胞，利用显微注射技术的小GTP酶的过度表达和分析所涉及的程序。

Tags

Small GTPasesMicroinjectionPlasmidsPolarized Epithelial CellsApical DomainBasolateral DomainTight JunctionsMDCK CellsCell PolarityRas SuperfamilyRalACdc42Rab8Rab10Rab13GDP-bound StateGTP-bound StateNucleotide Binding RegionsDominant NegativeDominant Active AllelesOverexpression

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