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器官型培养的全厚成年猪视网膜
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Neuroscienze
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JoVE Journal Neuroscienze
Organotypic Culture of Full-thickness Adult Porcine Retina
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器官型培养的全厚成年猪视网膜

DOI:
10.3791/2655-v

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08:17 min

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March 20, 2011

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, , ,
1Neurology and Neurosciences,University of Medicine and Dentistry of New Jersey – UMDNJ, 2Institute of Ophthalmology and Visual Science,University of Medicine and Dentistry of New Jersey – UMDNJ

Capitoli

  • 00:01Titolo
  • 00:50Make Custom Stand
  • 01:28Prepare Filter Papers for Culture
  • 01:37Isolate Retina from Pig Eye
  • 04:28Culturing of Isolated Retina
  • 06:03Sectioning of Retina
  • 07:31Immunohistochemistry
  • 07:58Discussion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一个成本效益的技术，七天为成年猪视网膜器官文化。简单地说，无菌滤纸用于解除神经视网膜视网膜色素上皮和地方感光一面向上插入一个定制的立场提出。

Tags

Organotypic CultureFull-thicknessAdult Porcine RetinaIn Vitro ModelsAnimal ExperimentsHuman Retinal InjuryDegenerative DiseaseCost-effective TechniqueAbattoirTrephine BladeNeural RetinaBruch’s MembraneRPE-choroid-sclera ExplantsSterile Filter PaperPhotoreceptor Side UpInsertCustom-made StandCulture MediumNative Retinal StructureMorphological StudiesPharmacological StudiesBiochemical Studies

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