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使用全血刺激试验的促炎性细胞因子IL -1β的精确和简单的测量

JoVE Journal
Medicina

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JoVE Journal Medicina

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

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使用全血刺激试验的促炎性细胞因子IL -1β的精确和简单的测量

DOI:

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06:29 min

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March 01, 2011

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Barbara Yang*¹, Tuyet-Hang Pham*², Raphaela Goldbach-Mansky, Massimo Gadina

¹Translational Immunology Section, Office of Science and Technology,National Institute of Arthritis and Musculoskeletal and Skin Diseases, ²Translational Autoinflammatory Disease Section, Office of the Clinical Director,National Institute of Arthritis and Musculoskeletal and Skin Diseases

Capitoli

00:05Titolo
01:29Collection of Whole Blood
02:02Preparation of Whole Blood
03:53Stimulation of the Whole Blood
05:15Cytokine Assay
05:34Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
06:08Conclusion

Summary

Traduzione automatica

我们描述了一个简单的免疫autoinflammatory表型的患者，来衡量生产促炎性细胞因子，如IL -1β生产。通过激活细胞与病原体相关分子模式，特别是与脂多糖，全血培养，细胞因子的分泌，可以方便地评估全血上清。

Tags

Pro-inflammatory Cytokine IL-1β Whole Blood Stimulation Assay Inflammatory Processes Immune Cells Cytokine Homeostasis Innate Immune System Pathogens Danger Stimuli Interleukin-1 (IL-1)Secretion Autoinflammatory Syndromes LPS Cellular Immune Responses PAMPs Gram-negative Bacterial Endotoxin

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