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Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
 

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

Article DOI: 10.3791/2686-v • 06:47 min • June 6th, 2011
June 6th, 2011 •
1, 1, 1, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, 2Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, 3Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University

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A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

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Glycans Zebrafish Embryos Metabolic Labeling Bioorthogonal Click Chemistry Chemical Reporter Strategy Azide-tagged Monosaccharides Alkyne-tagged Monosaccharides Glycan Biosynthetic Machinery Cell Surface Glycoconjugates Fluorescent Probes Affinity Probes Noninvasive Imaging Fucosylated Glycans Microinjection GDP-5-alkynylfucose (GDP-FucAl) Azide-conjugated Fluorophores Cu(I)-catalyzed Azide-alkyne Cycloaddition (CuAAC) Confocal Microscopy Sialic Acid-containing Glycans N-acetylgalactosamine-containing Glycans
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