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Invasion of Human Cells by a Bacterial Pathogen
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Invasion of Human Cells by a Bacterial Pathogen

Invasion of Human Cells by a Bacterial Pathogen

DOI:
10.3791/2693-v

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07:15 min

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March 21, 2011

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,
1Department of Biology and Biochemistry,University of Bath

Capitoli

  • 00:05Titolo
  • 01:14Introduction
  • 01:37Endothelial Cell Culture
  • 03:17Preparation of Bacteria
  • 04:07Invasion Assay
  • 05:48Expression of Fibronectin-binding Proteins on the Surface of S. aureus Confers the Ability to Invade Endothelial Cells
  • 06:52Conclusion

Summary

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A general protocol for the study of invasion of host cells by a bacterial pathogen, focusing on Staphylococcus aureus and human endothelial cells.

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InvasionHuman CellsBacterial PathogenStaphylococcus AureusStudyProtocolActin RearrangementCaveolaeHost CellsFlasks24-well PlatesThermanox CoverslipsBacteriaCulture MediumIncubationCO2PBSTriton X-100Cell LysisBacteria EnumerationSerial Dilution

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