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A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
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Biologia
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JoVE Journal Biologia
A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

DOI:
10.3791/270-v

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13:50 min

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August 30, 2007

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, ,
1Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital

Capitoli

  • 00:00Creating channel inlet and outlet
  • 00:06Introduction
  • 01:27Microfabrication of microfluidic device
  • 01:45Preparation of PDMS mixture
  • 02:42Patterning PDMS molds
  • 03:36Removing bubbles by vacuum
  • 04:21Assembly of microfluidic device
  • 04:55Cutting layers of microfluidic device
  • 06:10Bonding the microfluidic device layers
  • 08:19Coating channels with fibronectin
  • 09:08Experiment: monitoring apoptosis
  • 09:09Cell seeding
  • 11:31Application of reagents
  • 13:32Time lapse of apoptosis

Summary

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Traduzione automatica

We describe a protocol for the fabrication of microfluidic devices that can enable cell capture and culture. In this approach patterned microstructures such as grooves within microfluidic channels are used to create low shear stress regions within which cell can dock.

Tags

Microfluidic DeviceGroove PatternsCellular BehaviorMicrogrooved SubstrateFabricationPoly(dimethylsiloxane) (PDMS)Mouse Fibroblast CellsApoptosisOxidative StressAnnexin VPropidium IodidePhosphatidylserinePlasma MembraneReal-time ObservationInverted MicroscopeIncubation ChamberHigh-throughput Drug Screening

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