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Solid Plate-based Dietary Restriction in Caenorhabditis elegans
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JoVE Journal Biologia
Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

DOI:
10.3791/2701-v

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06:13 min

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May 28, 2011

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,
1Department of Internal Medicine, Division of Geriatric Medicine,University of Michigan, 2Department of Molecular and Integrative Physiology,University of Michigan

Capitoli

  • 00:05Titolo
  • 00:59Calibration Curve for Bacterial Concentrations
  • 02:00Preparation of Solid Plate-Based Dietary Restriction (sDR) Plates
  • 04:10sDR Experiments
  • 05:20Mean Lifespan: A Measure of Chronic sDR Treatments
  • 05:56Conclusion

Summary

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Here we present a protocol for performing solid plate-based dietary restriction in C. elegans with killed bacteria.

Tags

Solid Plate-based Dietary RestrictionCaenorhabditis ElegansFood Intake ReductionLifespan ExtensionAge-related DiseasesBody Weight DecreaseFertility DecreasePlasma Glucose DecreaseInsulin DecreaseIGF-1 DecreaseDietary Restriction (DR)Caloric Restriction (CR)Model OrganismEnvironmental ManipulationsGenetic ManipulationsLiquid Media PropagationSolid Agar In Petri PlatesDR ProtocolNGM Agar-based Plate

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