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Multivirus特异性T细胞的生成，预防/治疗异基因造血干细胞移植后的病毒感染
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
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Multivirus特异性T细胞的生成，预防/治疗异基因造血干细胞移植后的病毒感染

DOI:
10.3791/2736-v

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08:52 min

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May 27, 2011

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, , ,
1Center for Cell and Gene Therapy,Baylor College of Medicine

Capitoli

  • 00:05Titolo
  • 01:49DC Nucleofection
  • 04:01T Cell Stimulation
  • 06:18G-Rex Expansion of Multivirus-specific T Cells Generates High Numbers of Polyfunctional, Antigen-specific Effector T Cells
  • 08:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

一种快速，简便和成本效益的协议为代捐助衍生multivirus特异性CTL（rCTL）输注异体造血干细胞移植（HSCT）在发展巨细胞病毒，高级或EBV感染的风险收件人。这制造过程是按照GMP标准，并应确保实施专门的中心之外的更广泛的T细胞免疫。

Tags

Multivirus-specific T CellsViral InfectionsAllogeneic Hematopoietic Stem Cell TransplantEpstein Barr VirusCytomegalovirusAdenovirusAntigen Presenting CellsCytotoxic T LymphocytesGMP-compliant CTL Production ProtocolDendritic CellsDNA PlasmidsIL-4IL-7

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