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Capitoli
- 00:05Titolo
- 01:42Preparation of Bacterial Worm Food
- 03:04Large-scale C. elegans Liquid Culture
- 07:39Worm Collection and Preparation
- 10:46Fluorescence Analysis
- 11:30Representative Results
- 13:51Conclusion
A procedure for liquid-based culturing and dispensing of C. elegans strains expressing fluorescent reporter proteins is described that does not require expensive sorting equipment. This approach can be applied to numerous inducible C. elegans genes for drug discovery or biosensing of contaminants.
Tags
High-throughput ScreeningBiosensingFluorescent C. Elegans StrainsChemical ModulatorsBiological ProcessesCell Culture ModelsIn Vivo ScreeningWhole Animal ModelsDrug DevelopmentC. Elegans As A Model OrganismDrug Mode-of-action IdentificationCulturing And Dispensing ProtocolHigh-throughput Screening Of Chemical LibrariesDetection Of Environmental ContaminantsGene Expression AlterationFluorescence Microplate Reader
