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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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Biologia
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JoVE Journal Biologia
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DOI:
10.3791/2763-v

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13:32 min

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March 26, 2011

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, , ,
1Department of Integrative Oncology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Photography/Video Production, Multi-Media Services,BC Cancer Agency, 4Department of Pathology and Laboratory Medicine,University of British Columbia – UBC

Capitoli

  • 00:05Titolo
  • 00:38Paraffin Removal
  • 01:40Ethanol Rehydration
  • 02:58Tissue Digestion
  • 04:09DNA Clean Up
  • 07:12DNA Precipitation
  • 10:40DNA Quantification

Summary

Traduzione automatica

Traduzione automatica

This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

Tags

DNA ExtractionParaffin Embedded MaterialGenetic AnalysisEpigenetic AnalysisChromosomal RearrangementsCopy Number Gains And LossesDNA MethylationHigh-throughput Profiling TechnologiesMicroarraysSample QualitySpecimen CollectionFormalin-fixed And Paraffin Embedded (FFPE)Hospital ArchivesExtraction MethodXylene TreatmentEthanol WashesProteinase KLysis Buffer

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