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使用低温电子断层扫描和自动子断层扫描平均HIV包膜糖蛋白分子结构的测定
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
DOI:

07:29 min

December 01, 2011

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Capitoli

  • 00:05Titolo
  • 01:17Prepare Vitrified Virus Sample
  • 02:39Loading Samples into the Transmission Electron Microscope
  • 03:37Acquiring Cryo-electron Microscopy Data
  • 04:52Converting Microscopy Data into Structural Information that can be Visualized
  • 06:19Representative Results and Conclusion

Summary

Traduzione automatica

协议描述了高通量的方法,以确定使用低温电子断层扫描和三维图像处理的膜蛋白的结构。它涵盖了样品制备的详细信息,数据采集,数据处理和解释,最后一个有代表性的目标的方法,HIV - 1包膜糖蛋白的生产。这些计算程序设计的方式,使研究人员和学生远程工作和作出贡献的数据处理和结构分析。

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