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激活细胞凋亡的细胞色素的细胞质显微注射 C</em
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Biologia
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JoVE Journal Biologia
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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激活细胞凋亡的细胞色素的细胞质显微注射 C</em

DOI:
10.3791/2773-v

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07:42 min

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June 29, 2011

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, ,
1Department of Cell and Developmental Biology, Neuroscience Center,University of North Carolina , 2Curriculum in Neurobiology, Neuroscience Center,University of North Carolina

Capitoli

  • 00:05Titolo
  • 01:27Preparation of Protein Mixtures for Injection
  • 03:11Cytoplasmic Microinjection of Cytochrome c
  • 06:34Cytochrome c Injected Fibroblasts and Neurons
  • 07:24Conclusion

Summary

Traduzione automatica

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在这个协议中，我们描述直接胞浆内显微注射细胞色素<em> C</em>蛋白的成纤维母细胞和初级交感神经元。这种技术可以引进的细胞色素<em> C</em>蛋白进入细胞的细胞质和模仿释放细胞色素<em> C</em>从线粒体，细胞凋亡过程中发生。

Tags

ApoptosisProgrammed Cell DeathCytoplasmic MicroinjectionCytochrome CMitochondrial Intermembrane SpaceCaspasesCytotoxic StressesSignaling CascadesHolocytochrome CHeme-attached ProteinPost-mitochondrial RegulationCDNA ConstructsCytosolic CaspasesPurified Heme-attached Cytochrome C ProteinMitochondrial Cytochrome C Release

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