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确定基因表达概况 C。线虫使用微阵列和实时PCR
JoVE Journal
Biologia
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JoVE Journal Biologia
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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确定基因表达概况 C。线虫使用微阵列和实时PCR

DOI:
10.3791/2777-v

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10:27 min

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July 30, 2011

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, ,
1Department of Biological Sciences,Southwestern Oklahoma State University

Capitoli

  • 00:05Titolo
  • 01:12RNA Preparation
  • 03:32Microarray Hybridization
  • 05:32Microarray Analysis
  • 08:44Finding Gene Expression Changes in the vsm-1 Synaptogenesis Mutant
  • 10:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

进行微阵列分析，以确定基因的表达谱<em> C。线虫</em>，并实时PCR是用来验证和量化的微阵列数据。

Tags

Genetic Expression ProfilesC. ElegansMicroarrayReal-time PCRSynapsesPresynaptic Active ZonePostsynaptic TerminalNeurotransmittersReceptorsVSM-1Exocytosis RegulationSynaptic ConnectivitySynaptogenesisDouble-labeled Microarray AnalysisGene Expression ProfilesRNA IsolationCDNA Reverse TranscriptionDNA MicroarraysFluorescent Probe HybridizationMajor Sperm Protein Family (MSP)Nematode Oocyte MaturationOvulationPresynaptic Bouton Number And Size

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