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Vibratome切片增强保尔蒂哺乳动物器官的细胞构筑

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti

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Vibratome切片增强保尔蒂哺乳动物器官的细胞构筑

DOI:

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05:58 min

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June 17, 2011

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Katherine Shim

¹Department of Pediatrics, Children’s Research Institute,Medical College of Wisconsin

Capitoli

00:05Tile
01:22Dissection and Fixation of Inner Ears
04:07Mounting the Sections for Confocal Microscopy
04:53Antibody Staining and Confocal Microscopy of Pillar Cells in the Organ of Corti
05:28Conclusion

Summary

Traduzione automatica

一个简单的程序，切片，免疫组织化学和激光共聚焦显微镜Corti器的vibratome描述。此过程允许的罚款尔蒂哺乳动物器官的细胞结构的改善保存，因此可以准确定量的细胞类型。

Tags

Vibratome Sectioning Cytoarchitecture Mammalian Organ Of Corti Cellular Mosaic Mechanosensory Hair Cells Nonsensory Supporting Cells Cross-sectioning Histological Procedures Paraffin Sectioning Cryo-sectioning Immunohistochemical Staining Vibratome Sections Confocal Microscopy Immunohistochemical Analysis Mutant Organs Of Corti

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