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1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University
Capitoli
- 00:05Titolo
- 01:35Dissection of the Mouse Gastrointestinal Tract
- 02:56Whole Mount Sample Preparation and Immunostaining
- 05:10Cryosection Preparation and Immunostaining
- 07:44Representative Imaging Results
- 09:23Conclusion
Les cellules interstitielles de Cajal (CIC) sont les cellules pacemaker de gastro-intestinal (GI). Ils forment des réseaux complexes entre les cellules musculaires lisses et de fibres post-ganglionnaires neuronales pour réguler la contractilité IG. Ici, nous présentons les méthodes d'immunofluorescence transversale et l'ensemble de montage de visualisation des réseaux de la CPI murin.
Tags
VisualizationInterstitial Cells Of Cajal (ICC)MiceGastrointestinal (GI) TractPacemaker CellsSlow WavesPeristalsisEnteric Nervous SystemMotility DisordersKIT Receptor Tyrosine KinaseKIT ImmunostainingICC NetworkKIT ActivityNeoplastic TransformationGastrointestinal Stromal Tumor (GIST)ETV1 Survival FactorICC/GIST LineageTumorigenesisActivating KIT MutationsVisualization MethodsPreviously Published ProtocolsAnoctamin 1 (ANO1)Membrane MarkerImmunofluorescence
