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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
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Neuroscienze
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JoVE Journal Neuroscienze
Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

DOI:
10.3791/2803-v

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09:41 min

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July 20, 2011

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, ,
1Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research,University of Tuebingen , 2Graduate School for Cellular and Molecular Neuroscience,University of Tuebingen

Capitoli

  • 00:05Titolo
  • 01:04Sciatic & Dorsal Column Nerve Injury
  • 04:35Cross-linking
  • 06:14Immunoprecipitation and Elution
  • 08:23Increased Expression After Nerve Injury
  • 09:11Conclusion

Summary

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We present a method for chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury. The approach can be used to identify specific transcription factor binding sites and epigenetic modification of histone and DNA important for the regeneration of injured axons in both the peripheral and central nervous system.

Tags

Chromatin ImmunoprecipitationDorsal Root GangliaAxonal InjuryCentral Nervous SystemPeripheral Nervous SystemNeurite OutgrowthAxonal RegenerationTranscriptional ProgramsGene RegulationInjury Model SystemTranscriptional ActivityAnimal RemovalNerve FibersSciatic NerveSpinal CordTranscription Factor BindingHistone ModificationDNA ModificationChIP Protocol

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