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Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
 

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Article DOI: 10.3791/2813-v • 14:23 min • July 16th, 2011
July 16th, 2011 •
1, 1, 1,2
1The Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa

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An ex vivo protocol to generate mature human red blood cells from hematopoietic stem/progenitors is described. Additionally we describe an efficient lentiviral-delivery method to knockdown the transcription factor TAL1 in primary erythroid cells. The efficiency of lentivirus mediated gene delivery is demonstrated using GFP expressing viruses.

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Lentiviral-mediated Knockdown Ex Vivo Erythropoiesis Human Hematopoietic Stem Cells Cell Differentiation Gene Expression Transcription Factors Lineage-specific Genes Cell Lines Erythroid Cells Stages Of Differentiation Primary HSCs Erythroid Differentiation Cord Blood Bone Marrow Adult Peripheral Blood
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