Rivista
/
Biologia
/
High-throughput Yeast Plasmid Overexpression Screen
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
High-throughput Yeast Plasmid Overexpression Screen

High-throughput Yeast Plasmid Overexpression Screen

DOI:
10.3791/2836-v

•

08:57 min

•

July 27, 2011

•

,
1Neuroscience Graduate Group,University of Pennsylvania School of Medicine , 2Department of Cell and Developmental Biology,University of Pennsylvania School of Medicine

Capitoli

  • 00:05Titolo
  • 00:56Preparations for Yeast Transformation
  • 02:19Yeast Transformation Using a Liquid Handling Robot
  • 06:04Spotting Assay
  • 07:10Representative Results: Plasmid Overexpression Screen
  • 08:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.

Tags

High-throughputYeastPlasmidOverexpression ScreenSaccharomyces CerevisiaeGenetic ScreensGene FunctionLiquid Handling RobotArrayed LibraryNeurodegenerative Disease ProteinsCellular Phenotypes

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2836?article_type=v

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
check_url/it/2836?article_type=v

High-throughput Saccharification Assay for Lignocellulosic Materials
check_url/it/2836?article_type=v

High-throughput Purification of Affinity-tagged Recombinant Proteins
check_url/it/2836?article_type=v

RNA Secondary Structure Prediction Using High-throughput SHAPE
check_url/it/2836?article_type=v

High Throughput Microinjections of Sea Urchin Zygotes
check_url/it/2836?article_type=v

High-throughput Titration of Luciferase-expressing Recombinant Viruses
check_url/it/2836?article_type=v

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
check_url/it/2836?article_type=v

Recovery of Adult Zebrafish Hearts for High-throughput Applications
check_url/it/2836?article_type=v

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
check_url/it/2836?article_type=v

High Throughput Danio Rerio Energy Expenditure Assay

Read Article