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GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
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Neuroscienze
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JoVE Journal Neuroscienze
GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

DOI:
10.3791/2858-v

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09:59 min

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July 17, 2011

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, ,
1Department of Neuroscience,Uppsala University, Sweden

Capitoli

  • 00:05Titolo
  • 01:42Preparation of Brain Slices
  • 03:17Preparation of Pipettes
  • 04:00Patch-clamp Experiments
  • 08:07Representative Results
  • 09:30Conclusion

Summary

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We use the patch-clamp technique to measure GABA-activated single-channel currents (GABAA channels, GABAA receptors) and the synaptic and tonic currents they generate in neurons. Activation of the channels decreases neuronal excitability in health and disease 1,2,3,4.

Tags

GABA-activatedSingle-channelTonic CurrentsRat Brain SlicesGABAA ChannelsSynapsesPhasic CurrentsChloride ChannelPentameric GABA-gated ChannelChannel SubunitsFamiliesAlphasBetasFunctional ChannelSub-type SpecificNeuronal InhibitionPharmacological AgentsPatch-clamp TechniqueFunctional PropertiesHippocampal Brain SlicesSingle-channel CurrentsWhole-cell CurrentsGABA ConcentrationsDrugsIntra And Extracellular Factors

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