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Bioengineering

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JoVE Journal Bioengineering
Bacterial Immobilization for Imaging by Atomic Force Microscopy
 
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Bacterial Immobilization for Imaging by Atomic Force Microscopy

Article DOI: 10.3791/2880-v • 10:04 min • August 10th, 2011
August 10th, 2011 •
1,2, 3, 1,2, 1,4, 1,4
1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 3Department of Surgery, Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division, Oak Ridge National Laboratory

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Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).

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Bacterial Immobilization Atomic Force Microscopy High-resolution Imaging Imaging Cells Biomolecules Liquid Environment Scanning AFM Cantilever Tip Sample Immobilization Minimally Invasive Immobilization Porcine Gelatin Coating Negative Bacteria Electrostatic Interaction Factors Affecting Immobilization Microbial Cell Imaging
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