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Induction and Testing of Hypoxia in Cell Culture
JoVE Journal
Biologia
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JoVE Journal Biologia
Induction and Testing of Hypoxia in Cell Culture

Induction and Testing of Hypoxia in Cell Culture

DOI:
10.3791/2899-v

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07:01 min

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August 12, 2011

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,
1Center for Cell and Gene Therapy,Baylor College of Medicine

Capitoli

  • 00:05Titolo
  • 01:10Inducing Hypoxia Using CoCl2 Solution and a Modular Incubator Chamber
  • 03:04Evaluating Hypoxia
  • 04:57Measuring Luciferase Activity
  • 05:35Hypoxia Detection Results
  • 06:31Conclusion

Summary

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Here we propose simple methods to induce hypoxia in cell cultures and simple tests to evaluate the hypoxic status of the cultures.

Tags

InductionTestingHypoxiaCell CultureOxygen ReductionOxygen TensionSupplyBlood VesselsAnemiaConsumptionProliferation RatePhysiologicPathologicSolid CancersRheumatoid ArthritisAtherosclerosisAdaptation AbilityHypoxia Inducible Factor Alpha (HIF)StabilizationAngiogenesisOxygen TransportProtein DetectionScreening

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