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无试剂检测核酸，蛋白质和小分子的电化学DNA生物传感器的制备
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
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无试剂检测核酸，蛋白质和小分子的电化学DNA生物传感器的制备

DOI:
10.3791/2922-v

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13:15 min

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June 01, 2011

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, , ,
1Department of Chemistry and Biochemistry,University Of California Santa Barbara, 2Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering,University Of California Santa Barbara

Capitoli

  • 00:05Titolo
  • 01:28Preparation of Chemically Modified Probe DNA
  • 02:48Sensor Preparation
  • 05:30Sensor Testing, DNA Detection
  • 07:57Sensor Testing, Antibody Detection
  • 09:47Sensor Testing, Small Molecule Detection
  • 11:24Representative Results obtained using Electrochemical-DNA Biosensors
  • 12:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

无试剂，“E - DNA”的传感器，电化学传感器表现良好，即使在血液和其他复杂的矩阵直接挑战时，已适应了广泛的核酸，蛋白质和小分子分析物的检测。在这里，我们介绍了这种传感器的制造和使用的一般程序。

Tags

Electrochemical-DNA BiosensorsReagentless DetectionNucleic AcidsProteinsSmall MoleculesBedside TestsRapid DetectionQuantitative DetectionClinical SamplesEnvironmental SamplesE-DNA BiosensorsTarget DNA Sequence DetectionPolymerase Chain Reaction MixtureHIV-specific Antibody DetectionDrug Cocaine Detection

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