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Biochemical Measurement of Neonatal Hypoxia
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JoVE Journal Medicina
Biochemical Measurement of Neonatal Hypoxia

Biochemical Measurement of Neonatal Hypoxia

DOI:
10.3791/2948-v

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13:13 min

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August 24, 2011

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, , , ,
1Division of Biochemistry, Department of Basic Sciences,Loma Linda University, 2Division of Physiology, Department of Basic Sciences,Loma Linda University

Capitoli

  • 00:05Titolo
  • 01:37Sample Collection and Processing
  • 02:28HPLC Measurement of Purines
  • 03:54Gas Chromatography-Mass Spectrometry (GC/MS) Measurement of Allantoin
  • 06:18GC/MS Sample Preparation for Measurement of MDA
  • 07:44HPLC Measurement of Xanthine Oxidase
  • 10:40Biochemical Measurement of Neonatal Hypoxia: Representative Results
  • 12:55Conclusion

Summary

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A method is described to measure biochemical markers of neonatal hypoxia-ischemia. The approach utilizes high pressure liquid chromatography (HPLC) and Gas Chromatography Mass Spectrometry (GC/MS).

Tags

Neonatal HypoxiaInadequate Blood PerfusionLack Of OxygenAdenosine Triphosphate ProductionOxidative PhosphorylationHigh Energy Phosphate BondsAdenosine DegradationInosineHypoxanthineXanthineUric AcidXanthine OxidoreductaseXanthine DehydrogenaseXanthine Oxidase (XO)Hydrogen PeroxideReactive Oxygen Species (ROS)AllantoinMalondialdehyde (MDA)UricaseOxidative Stress

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